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recombinant clones中文是什么意思
- 中文翻譯
- 造句
- 重組體克隆
- recombinant n. 【遺】重組器官，復(fù)合器官。
- clone n. 1.【生物學(xué)】純種細(xì)胞，無性系。 2.復(fù)制品；(幾 ...
- clones 克朗斯; 克隆斯; 汽車人克隆戰(zhàn)士
- attack of the clones 復(fù)制人全面進(jìn)攻; 克隆人戰(zhàn)爭; 星球大戰(zhàn)前傳ii復(fù)制人的進(jìn)攻; 星球大戰(zhàn)前傳ii克隆人的進(jìn)攻
- identification of clones 無性系鑒定
- overlapping clones 重疊克隆
- proved clones 經(jīng)過鑒定的無性系
- star wars attack of the clones 克隆人進(jìn)攻
- star wars episodeiiattack of the clones 星戰(zhàn)前傳ii克隆人的進(jìn)攻
- star wars2 attack of the clones 星戰(zhàn)前傳2克隆人的進(jìn)攻
- within-species clones 種內(nèi)無性系
- alteplase recombinant 重組阿替普酶
- bacterial recombinant 細(xì)菌重組
- biparental recombinant 二親重組體
- dna recombinant dna重組
- library of recombinant 重組基因文庫
- patch recombinant 片段重組體
- plasmid recombinant 重組體質(zhì)粒
- recombinant bacteria 重組細(xì)菌
- recombinant chromosome 重組染色體
- recombinant dna 【生物化學(xué)】重組脫氧核糖核酸。
- recombinant enzymes 重組酶
- recombinant genome 重組體基因組
- recombinant immunotoxin 重組免疫毒素
- recombinant joint 重組接點
- The pcr product was cloned into pmd18 - t vector . the positive recombinant clone was identified by pcr and endonuclease digest
提取重組質(zhì)粒經(jīng)pcr鑒定和酶切鑒定后，對插入片段進(jìn)行序列測定及分析。 - Recombinant cloning vector of pgem - hc and pgem - ha were constructed by ligating the hc and ha genes into pgem t east vector and transformating into jm109 . 3
分別將目的基因hypoderminc和hypodermina與克隆載體( pgemteasy )連接并轉(zhuǎn)化到宿主菌jm109中，構(gòu)建了重組克隆載體pgem - hc和pgem - ha 。 - Then the linked products were transformed into the high competent cell of e . coli dh5a . based on - complementation of the detective - galactosidase , positive recombinant clone were screened from x - gal plate
從引物和基因序列的比對分析結(jié)果看， zhyf006序列與上下游引物的配對比例分別為s4 - In this experiment , ri gene was amplified by pcr method from recombinant cloning vector pt7 - ri , which had been constructed by dr . yu xiu - ping in this laboratory and sequenced by takara biotechnology ( dalian ) co . , ltd .
于秀平博士已經(jīng)構(gòu)建了ri的克隆載體pt7 - ri ，測序表明與已知人胎盤ri基因的cdna序列完全相同。本文的工作是在此基礎(chǔ)之上完成的。 - In this study , we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene . about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector . recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing . next , we construct recombinant plasmid pproex ? t - il - 2 . the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector . the recombinant plasmid pproex ? t - il - 2 was transformed into e . coli dh5a and the bacteria was induced with iptg . it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e . coli dh5a . the expression level was up to 30 % of the total bacterial proteins . the purified protein was used to prepare the antibody against chicken il - 2 protein
經(jīng)酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎(chǔ)上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達(dá)載體pproex ~ ( tm ) ht中，構(gòu)建重組表達(dá)質(zhì)粒并進(jìn)行確證性序列測定，重組質(zhì)粒測序結(jié)果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達(dá)載體pproex ~ ( tm ) ht的目的位點。重組質(zhì)粒轉(zhuǎn)化受體菌dh5后用iptg于37進(jìn)行誘導(dǎo)培養(yǎng)， sds - page和westernblot分析顯示，表達(dá)的雞il - 2融合蛋白分子量約為18kda ，表達(dá)的融合蛋白經(jīng)薄層掃描發(fā)現(xiàn)目的蛋白表達(dá)量約占菌體蛋白的30 。 - We sequence the inserted gene fragment of the indentified recombinant clone . the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly . but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu
隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結(jié)果為： as基因開放讀碼框與表達(dá)載體的讀碼框正確匹配相連，但在其kringle4區(qū)相當(dāng)于編碼plg的lys ~ ( 414 )密碼子aaa的第一位堿基由a突變?yōu)間 ，導(dǎo)致相應(yīng)的氨基酸殘基突變?yōu)間lu 。 - The gene was cloned into puc19 vector and identified with pcr and eco ri + hind iii digestion . then , one of the positive recombinant clone was sequenced and analysed . the result showed that its sequence was 35 % and 32 % identical to equine ifn - and human ifn - respectively , but it only shared 15 % homology with chicken type i ifn
序列分析表明，該基因閱讀開放框架由492個核苷酸組成，與馬和人ifn -基因序列的同源性分別為35和32 ，與雞i型干擾素基因的同源性僅為15 ，與國外報道的雞ifn -基因序列完全一致。 - Using enterobacter cloacae b8 , the mutated strains b8b and b8f , and the recombinant clones pb and pf , we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system . the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank
以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質(zhì)粒pb 、 pf為基礎(chǔ)，對陰溝腸桿菌b8菌株拮抗相關(guān)的b和f基因片段進(jìn)行序列分析。
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